![]() ![]() Tris buffers stored at room temperature will have fluctuations in pH after extended storage (see: this article). Before use, verify that the pH is correct. Please note: We recommend making the buffer, aliquotting it, and then storing it at 4C. If using an alternative, please keep in mind it would be untested and we cannot guarantee assay performance. pH 8), but this may result in a decline in assay sensitivity depending on sample quality and type. It is advised to adjust the buffer pH to 9. ![]() Additional reagents in the buffer besides Tris and EDTA are undesirable and may impact assay performance. If you’d like to use an alternative commercially available TE buffer for decrosslinking, check that there are no additives or detergents. EDTA chelates Mg2+ and other divalent metals ions - (inhibits DNAse and. The recipe is in the Visium for FFPE H&E and Immunofluorescence Staining Demonstrated Protocols. Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. Question: I can’t purchase the recommended TE buffer, pH 9.0 (Genemed, PN: 10-0046), are there any alternatives that could be used?Īnswer: If you cannot purchase the recommended buffer, we would advise making it using Tris Base and EDTA. ![]()
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